RNA-basierte Regulation der Genexpression: künstliche Genschalter

Abstract RNA-based gene control mechanisms pose an elegant and straightforward way to switch on, off, or fine-tune transgene expression without the need for expressing regulatory proteins. A small molecule effector binds directly a ligand-binding aptamer RNA structure thereby modulates of associated target gene. We established genetic switches based on regulation self-cleaving ribozymes polyadenylation that allow in bacteria, yeast, human cell lines Caenorhabditis elegans robust dose-dependent manner.